When the series began, Peter Parker had been Spider-Man for one year. He had saved lives and fought supervillains, but he was still in the process of learning how to be a superhero. Nick Fury of S.H.I.E.L.D. offered Peter the chance to train to be a real superhero and become "The Ultimate Spider-Man". However, Peter had to first learn how to work with a team of four fellow teenage superheroes: Nova, White Tiger, Iron Fist and Power Man.
In Season 2, following the fight with the Green Goblin which resulted in the destruction of the S.H.I.E.L.D. Helicarrier, Spider-Man's team ended up staying at Peter Parker's house. Season 2 feature the first appearances in this series of Lizard, Electro, Rhino, Kraven the Hunter, Hawkeye, the Sinister Six and Iron Patriot.
In the upcoming Season 3, different corners of the Marvel Universe showing new heroes such as Ka-Zar of the Savage Land, Agent Venom, Cloak & Dagger and Amadeus Cho & the Spider-Verse story which will feature various versions of Spider-Man including Miles Morales.